Anonymous Coward:
Excellent question, and cavergirl supplied you with a good answer. Similarly, researchers often get confused. Just minutes ago, I received the updated WNS map from Cal Butchkoski. You'll note he has not only added Indiana as a site, but has changed the terminology to "suspect" to conform with the USGS NWHC Classification system.
The short answer for the Indiana report is that the fungus was confirmed, but no histology, therefore not WNS "confirmed." By the way, I have from two sources that we may hear more shortly from Indiana, which wouldn't surprise me.
Here's the new map, and Cal's comments below:
Click to enlarge
Hi All,
Unfortunately the state of Indiana announced the detection of WNS in a Washington Co., cave yesterday. I’ve classed it as “suspect” since it appears no histo results are back yet. There will likely be more reports from Indiana.
I’ve changed the “Likely” category to “Suspect” to match with the NWHC case definition categories recently announced:
Suspect WNS:
To identify a bat as suspect for WNS, one of the following must be true:
• Field signs are suggestive of WNS ANDWNS was previously confirmed in the county or in an adjacent county. Further diagnostics (PCR, culture, fungal tape and histopathology) were either not performed or are negative.
• A bat is PCR positive meaning that DNA from G. destructans is present although the viability of the organism is unknown. Field signs are not required. No histopathology was performed or is negative.
• A bat is culture positive meaning there is viable G. destructans present. Field signs are not required. No histopathology was performed or is negative.
• Fungal tape strip of bat fur or skin is positive for G. destructans-like conidia. Visible fungus is required. No histopathology was performed or is negative.
Confirmed positive for WNS:
• Confirmed positive bats are those that fulfill histopathologic criteria for the disease. These criteria require the identification of a specific pattern of fungal colonization in the epidermis which may extend to invasion of the dermis and connective tissue. Histopathology can also support the presence/identity of G. destructans if distinctive conidia are observed. Field signs, PCR, fungal tape strip, and culture can be negative for bats that fulfill the histopathologic criteria for confirmed WNS. Follow-up PCR/DNA sequencing or fungal culture should be considered to confirm the identity of the organism in geographic regions with no prior or unknown history of WNS.
I regretfully welcome Indiana to the riddled and rattled WNS raft.
Cal Butchkoski
Wildlife Diversity Biologist
PA Game commission